Biological
Technology Certificate Program
at Pasadena City College
Biotech Lab Skills Courses
- Validation, calibration and measurement formats
- Use of top-loading balances, analytical balances and double pan balances
- Use of pipettors, micropipettors
- Making solutions (molarity, percent )
- pH techniques and production of buffers
- Use of microfuges and clinical centrifuges
- Sterile technique and media production
- Use of autoclaves and inoculation techniques: streaks and swabs
- Growth curves (mid-log phase and overnight cultures), use of shaker incubator
- Use of microscopes and photography using the microscope
- Use of digital camera for documentation
- UV-Vis Spectrophotometer for cell counts, absorption spectra and standard curves
- Storage and inventory protocols (-80oC, -20 oC, 4 oC, RT)
- QA and QC general strategies
- Genomic DNA Extraction
- Horizontal gel electrophoresis, ethidium bromide staining and gel documentation
- Restriction digests, ligations, transformations, selective media and plasmid minipreps
- Laminar flow hood protocols
- Hazardous material storage, fume hoods, MSDS sheets and Internet MSDS
- Production of a skills-based resume
- Production of proper lab notebook documentation (Excel, digital images, etc)
- Production and analysis of Southern blots, PAGE gels, Western blots.
- Column chromatography (affinity, ion-exchange, gel filtration).
- PCR, DNA sequencing, ELISA
- Use of NCBI Databases
- Animal cell culture, immunostaining, digital analysis, poster session
Prerequisite - Biology 102B or Biology 102C
Work experience in industrial situation appropriate to students experience in Biology 102B and/or Biology 102C.
Biology 38 - Cell & Molecular Biology
Stem cell culture
Staining techniques
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©Pasadena City College,
1570 E. Colorado Blvd., Pasadena, CA
91106, 626-585-7123
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